Coding

Part:BBa_K1132014:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)

Promoter (OmpR, positive) followed by RFP protein generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 692
    Illegal AgeI site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The monomeric RFP is a Red Fluorescent Protein with an excitation peak at 584 nm and an emission peak at 607 nm. The RFP is positively regulated by OmpR-controlled promoter. Phosphorylated OmpR binds to the operator sites and activates transcription of RFP.

This is a test Biobrick for the Red light responsive system (Part:BBa_K1132013 and Part:BBa_K1132015) with the RFP protein as output. EnvZ phosphorylates OmpR to OmpR-P that activate the promoter. It must be used in E.coli deficient in wild-type EnvZ.



Source

later

References